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KMID : 0620920230550081831
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Experimental & Molecular Medicine
2023 Volume.55 No. 8 p.1831 ~ p.1842
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A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung
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Lee Woo-Chan
Lee Se-Yoon
Yoon Jung-Ki
Lee Da-Kyung
Kim Yu-Ri
Han Yeon-Bi
Alexandre Mebazaa
Benoit Gallix
Park Young-Jun
Park Kyung-Hyuk
Cha Bu-Kyoung
Choi Jae-Yong
Kim Ju-Hyun
Ha Na-Young
Kim Kwhan-Mien
Cho Suk-Ki
Cho Nam-Hyuk
Tushar J. Desai
Chung Jin-Haeng
Lee Joo-Hyeon
Kim Jong-Il
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Abstract
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We present an in-depth single-cell atlas of in vitro multiculture systems on human primary airway epithelium derived from normal and diseased lungs of 27 individual donors. Our large-scale single-cell profiling identified new cell states and differentiation trajectories of rare airway epithelial cell types in human distal lungs. By integrating single-cell datasets of human lung tissues, we discovered immune-primed subsets enriched in lungs and organoids derived from patients with chronic respiratory disease. To demonstrate the full potential of our platform, we further illustrate transcriptomic responses to various respiratory virus infections in vitro airway models. Our work constitutes a single-cell roadmap for the cellular and molecular characteristics of human primary lung cells in vitro and their relevance to human tissues in vivo.
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KEYWORD
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Genetic databases, Stem-cell differentiation, Transcriptomics
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FullTexts / Linksout information
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Listed journal information
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